Quantitative (calibrated) Anti-human Tetraspanin vTag™ antibody staining of human platelet-derived EVs (MESF) vs size (nm).
Quantitative (calibrated) Anti-human Tetraspanin vTag™ antibody staining of human platelet-derived EVs (MESF) vs size (nm).
Validation of anti-human tetraspanin antibody on positive control EVs (human platelet-derived EVs).
Validation of anti-human tetraspanin antibody on EVs derived from U87 cells.
Validation of anti-human tetraspanin antibody on negative control sample (Lipo100™)

vTAG™ ANTI-HUMAN TETRASPANIN ANTIBODY
NO WASH, QUANTITATIVE TETRASPANIN MEASUREMENT BY vFC™

$215.00 – $525.00

Conjugate(s): PE
Est. Limit of Detection: 30 Molecules per EV

 

Cargo analysis using vTag™ antibodies should be accompanied by a vesicle specific analysis method such as vFC™. vTag™ antibodies bundled with the vFC™ assay will be discounted 25%.
Certificate of Analysis
TDS MSDS
SKU: CBS5 Category:

Description

Product Attributes

Tested Reactivity: This antibody is a cocktail of anti-Human mAbs against human CD9, human CD63, and human CD81.
Other Published Reactivity: Non-human primates, including cynomolgus, and rhesus.
Conjugate: PE (see dropdown at the top for available options).
Sensitivity: Limit of detection is estimated at 10-30 molecules per vesicle in vFC™ for the PE conjugate and is instrument dependent. Change selection above for LOD of the antibody conjugated to a different fluorophore. LOD is estimated using antibody binding beads and will vary depending upon sample, instrument, and other experimental conditions.
Application: Optimized for vFC™ with no wash steps required.
Clonality: Cocktail of 3 monoclonal antibodies.
Clone: Multiple
Host and Isotype: Mouse IgG1, kappa.
Positive Control Sample: EVs from human platelets.
Localization of Staining: Vesicle Surface.
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Concentration: 5 µl/test.
Purification Method: Affinity purified during antibody production, additionally purified during conjugation to remove free dye and unbound antibody.
Immunogen: Multiple.
Storage Conditions: Store undiluted between 2°C and 8°C. Protected from prolonged exposure to light. Do not freeze. Stable for 1 year when properly stored.

Protocols

Human Tetraspanin Immunostaining in vFC™?

Cellarcus’ anti-human tetraspanin antibody is included with the vFC™ assay.  Protocols for characterization of extracellular vesicles can be found on the vFC™ assay page. Protocol 2 describes immunofluorescent based measurement of cargo including human tetraspanins.  It should be run after completion of instrument setup (protocol 0) and sample characterization (protocol 1) to ensure compliance with all current guidelines.

Tetraspanin Background Information

Tetraspanins are common surface proteins on EVs.  The majority of EVs express predominantly CD9, CD63, and/or CD81 although expression patterns differ on EVs derived from different cell lines.  More information on tetraspanins coming soon!

Datasheets and Documentation

Certificate of Analysis

MSDS

Additional information

Size

20 Tests, 100 Tests
Conjugate

PE, APC

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NO WASH, QUANTITATIVE TETRASPANIN MEASUREMENT BY vFC™
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Quantitative (calibrated) Anti-human Tetraspanin vTag™ antibody staining of human platelet-derived EVs (MESF) vs size (nm).
vTAG™ ANTI-HUMAN TETRASPANIN ANTIBODY
NO WASH, QUANTITATIVE TETRASPANIN MEASUREMENT BY vFC™
$215.00 – $525.00
https://www.cellarcus.com/wp-content/uploads/2023/01/Tetraspanin-mix-PE-antibody-vFC-PLT-EV-1P.pnghttps://www.cellarcus.com/wp-content/uploads/2023/01/Tetraspanin-mix-PE-antibody-vFC-U87-EV-1P.pnghttps://www.cellarcus.com/wp-content/uploads/2023/01/Tetraspanin-mix-PE-antibody-vFC-293T-EV-1P.png
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