Product box for vFC - EV (Exosome, Microvesicle, or other Lipid Vesicle) Characterization Assay

vFC™ VESICLE FLOW CYTOMETRY EV ANALYSIS ASSAY
 FOR COUNTING AND SIZING VESICLES IN BIOFLUIDS, MEDIA, OR BUFFER

$450.00$2,100.00

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Description

Sample Type: Suitable for characterization of extracellular vesicles directly from cell culture supernatant or biofluids such as plasma, urine, or CSF. Can also be used to analyze EVs separated from complex samples using various techniques.  See kit manual for sample type specific assay considerations.

Species: Human. Although most reagents are not species specific, certain controls restrict the use of this kit to human samples.

Limits of Detection: vFC™ is suitable for the enumeration of vesicles down to 50nm from background. By following our staining protocols and using vTag™ monoclonal antibodies, surface antigen detection down to 10 molecules per vesicle is possible. Limit of detection is instrument specific. See product manual for additional information.

Cargo Measurement: vFC™ enables quantitative cargo measurement in a no-wash format using fluorescently labeled antibodies. We recommend vTag™ antibodies which perform optimally and have been appropriately titrated for vFC™.

Instrument Requirements: vFC™ can be run on sensitive, commercially available flow cytometers including the Beckman Coulter CytoFLEX® and the Luminex CellStream® and ImageStream®.

Sample Storage: Care should be taken to keep assay and storage conditions consistent and report all procedures. If vesicles cannot be analyzed fresh, we recommend storing at -80°C and aliquoting to avoid unnecessary freeze/thaw cycles.

Kit Storage: CAUTION! Multiple storage temperatures required. See product manual for instructions.

What are the limitations of the assay?

vFC™ assay (#CBS4) is intended for the analysis of vesicles from cell culture in supernatant or buffer. It is also useful for analyzing vesicles separated from biofluids. It can detect vesicles down to 50nm on currently available instrumentation. Limit of detection of surface markers per vesicle is approximately 10 molecules (depending on the instrument and the vTag™ antibody used). We have characterized limits for several instruments. Please contact us for instrument specific sensitivity assessments.

Why am I not seeing events from the Lipo100™ control sample?

There are several possible reasons for this issue. First, ensure that the instrument you’re using is suitable for vFC™. Next, make sure that the instrument has been properly set up and qualified. For instrument setup procedures see the instrument setup and configuration document under the datasheets and documentation tab. Finally, double check that samples are being properly diluted. Pay careful attention to details such as the plate being used for dilutions as certain plastics may bind vesicle and significantly impact EV counts. For additional support, chat with us by clicking on the icon at the bottom right of the window.

Why am I seeing high background events?

High number of background events can arise from multiple sources. Ensure proper dilution of samples and only run vesicles that are appropriate for this kit. Also, rarely carryover from samples may lead to background which is especially noticeable in blank samples. This is most commonly observed after running small beads. Run a daily clean cycle prior to continuing analysis. If issues continue, run beads diluted 1:10 in cleaning solution in the future.

Certificate of Analysis

MSDS

Additional information

Size

96 Wells, 5 x 96 Wells

EV Marker (Antibody)

Anti-Human TS Cocktail (CD9, CD63, CD81) [PE], Anti-Human TS Cocktail (CD9, CD63, CD81) [PE-Cy7], Anti-Mouse TS Cocktail (CD9, CD63, CD81) [PE], Anti-Mouse TS Cocktail (CD9, CD63, CD81) [PE-Cy7], Other, None

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 FOR COUNTING AND SIZING VESICLES IN BIOFLUIDS, MEDIA, OR BUFFER

Product box for vFC - EV (Exosome, Microvesicle, or other Lipid Vesicle) Characterization Assay
vFC™ VESICLE FLOW CYTOMETRY EV ANALYSIS ASSAY
 FOR COUNTING AND SIZING VESICLES IN BIOFLUIDS, MEDIA, OR BUFFER
$450.00$2,100.00
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