Cellarcus was founded by Dr. John Nolan, a leading researcher in extracellular vesicle analysis technologies. Our mission is to provide tools and resources to generate accurate and reproducible data needed to move vesicle research forward. Our assays and reagents are supported by our expert technical team to help you overcome challenges inherent to measuring these small particles. Have a question? Ask us!
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Details for storing our reagents can be found on our product pages. They are also provided on datasheets that are shipped with the products.
Expiration dates are listed on the certificates of analysis which is sent with the product and can be downloaded with a lot number from the product webpage. They are also listed on the vial label.
Our assays such as vFC™ have been validated on several commercially available flow cytometers. While we have the most experience with the Beckman Coulter CytoFlex™. We are pleased with early test results on the Acea Novocyte® Quanteon™, the Amnis ImageStream™, and the Amnis CellStream™. If you don’t have access to an instrument on this list, we can make recommendations on instrument configuration if you’re considering new instrumentation for you lab. We offer necessary controls such as vCal™ Rainbow nanoBeads and reference preps of common vesicles to aid in evaluating instrument performance.
Limit of detection for assays and antibodies are listed both on the product web page and on the data sheet shipped with the assays and antibody panels. Currently there are three types of limits of detection to consider for vesicle assays:
(1) Concentration of vesicles
(2) Size of vesicles
(3) Number of molecules per vesicle
As instrument sensitivity improves we expect all parameters to improve. Currently, for most products, vesicles down to approximately 70nm can be characterized. A minimum of 30 molecules/vesicle of a given marker are required for vTag™ cargo detection.
Guidelines for studying and reporting data on vesicles are updated annually by a working group from the international society of extracellular vesicles. A link to the latest publication and our summary of recommendations can be found on our MISEV guidelines page.