Cellarcus was founded by Dr. John Nolan, a leading researcher in extracellular vesicle analysis technologies. Our mission is to provide tools and resources to generate accurate and reproducible data needed to move vesicle research forward. Our assays and reagents are supported by our expert technical team to help you overcome challenges inherent to measuring these small particles. Have a question? Ask us!
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Details for storing our reagents can be found on our product pages. They are also provided on datasheets that are shipped with the products.
Expiration dates are listed on the datasheets and certificates of analysis. They are also listed on the vial label and on the products’ web page.
While our assays work on commercial flow cytometers, they don’t work on every commercial flow cytometer. Vesicles are extremely small and difficult to measure. While our assay makes vesicle analysis possible, it does require sensitive instrumentation for optimal performance. Currently, we have tested the assays on several instruments, both custom and commercially standard. We are pleased with the performance of the Beckman Coulter CytoFlex, the Amnis ImageStream™ and CellStream™, and the Acea Novocyte Quanteon™ platforms. We are happy to make recommendations on instrument configuration or provide information and protocols to help evaluate instrument suitability. We are currently in the process of testing other instruments and will add recommendations once we are confident in the data.
Limit of detection for assays and antibodies are listed both on the product web page and on the data sheet shipped with the assays and antibody panels. Currently there are three types of limits of detection to consider for vesicle assays:
(1) Concentration of vesicles
(2) Size of vesicles
(3) Number of molecules per vesicle
As instrument sensitivity improves we expect all parameters to improve. Currently, for most products, vesicles down to approximately 70nm can be characterized. A minimum of 30 molecules of a given marker are required for immunofluorescent detection.
Guidelines for studying and reporting data on vesicles are updated annually by a working group from the international society of extracellular vesicles. A link to the latest publication and our summary of recommendations can be found on our MISEV guidelines page.