Cellarcus Biosciences

Instrument Qualification

Acea Quanteon, Beckman Coulter CytoFLEX, Amnis CellStream

Start here. Follow our vCal™ Nanorainbow Bead instrument evaluation and QC protocol to ensure your instrument is suitable and running optimally to perform vFC™ analysis of your EV samples.

See Protocol 0

Size and Count Vesicles

Extracellular Vesicle Analysis via VFC demonstrates highly reproducible data on vesicles derived from MSCs.

vFC™ protocol 1 describes the procedure for obtaining sizes and counts of vesicles when examining sample types that you haven’t previously characterized and where concentration is unknown. The protocol details our recommend methods for running serial dilutions of EV samples to determine vesicle concentration.

See Protocol 1

Measure Vesicle Cargo

vFC™ Staining of vesicles from cells with established markers. (a) Liposomee standard without surface marker expression, (b) Plot of PE intensity vs vesicle size for vesicles from frst cell type shows positive shift for known marker of cell type I and no signal for cell type II marker, (c) Plot of PE intensity vs vesicle size for vesicles from cell type II show positivity for cell type II marer with no signal for cell type I marker.

Once you’ve identified an appropriate dilution, move on to characterizing EVs by surface marker expression. This protocol details methods for quantitative immunofluorescent staining of your EV samples using vFC™ and appropriately validated antibodies (we recommend using vTag™ mAbs which are validated for use in vFC™).

See Protocol 2

Stay in the Loop

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