Determine EV Concentration
Instrument calibration workflows and standards combine with our EV specific measurements in the vFC™ assay kit for accurate determination of EV concentration.
Determine EV Size
vFC™ determines EV size from fluorescence of a membrane stain, vFRed™. It is simpler and relies on fewer assumptions than other flow cytometric methods.
Quantify EV Cargo
vFC™ can be paired with validated, optimized vTag™ antibodies for multiplexed cargo measurement on single EVs. Cargo measurement is quantitative.Instrument Qualification
Start here. Follow our vCal™ Nanorainbow Bead instrument evaluation and QC protocol to ensure your instrument is suitable and running optimally to perform vFC™ analysis of your EV samples.
See Protocol 0Size and Count Vesicles
vFC™ protocol 1 describes the procedure for obtaining sizes and counts of vesicles when examining sample types that you haven’t previously characterized and where concentration is unknown. The protocol details our recommend methods for running serial dilutions of EV samples to determine vesicle concentration.
See Protocol 1Measure Vesicle Cargo
Once you’ve identified an appropriate dilution, move on to characterizing EVs by surface marker expression. This protocol details methods for quantitative immunofluorescent staining of your EV samples using vFC™ and appropriately validated antibodies (we recommend using vTag™ mAbs which are validated for use in vFC™).
See Protocol 2