HUMAN PLATELET DERIVED EXTRACELLULAR VESICLES
$275.00 – $1,300.00
387 in stock
Vesicle Size:140 nm (Range: 80-210nm)
Certificate of Analysis
Are these the right standards for my application?
Human platelet derived extracellular vesicles from Cellarcus provide a consistent, rigorously characterized reference sample to baseline assays over multiple runs. These samples are also suitable for researchers examining platelet derived exosomes, microvesicles, or other EVs.
Cellarcus’ Human Platelet Derived Extracellular vesicles are a reference sample prepared from platelets obtained by a standard platelet enrichment protocol. Platelet vesiculation is induced by Ca2+ selective ionophore. The resulting vesicles are isolated via centrifugation and provided at a concentration of 1 x 107 vesicles/µL as measured by Vesicle Flow Cytometry (vFC™). Unlike traditional applications like NTA or TRPS, it is vesicle specific. The specificity of the measurement is a key driver behind 5-fold improved assay variability compared to these other applications. Concentrations measured by vFC™ are generally 1-2 log units lower than other applications because it is counting only vesicles, not all particles. vFC™ analysis enables characterization of these vesicles samples by size, concentration and cell surface cargo.
Below Limit of Detection: (none tested).
Vesicle flow cytometry requires a sufficiently sensitive instrument and vesicle analysis procedure. We provide simple, accurate, reproducible vesicle staining assays for this purpose (coming soon). At 1 x 107 vesicles/µL this reference sample uses 5 µl/test for VFC analysis. The sample concentration requirement is the same for NTA or RPS, however, volumes required for assay on many instruments are up to 10-fold higher. As much as 50 µl of this EV reference sample may be required for these assays. Exact volume(s) of reagents should always be empirically determined.
50 µl, 100 µl, 500µl