Vesicle Flow Cytometry data showing size and number of vesicles in platelet derived EV reference sample.



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Source:Human Platelets
Vesicle Size:140 nm (Range: 80-210nm)
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Are these the right standards for my application?

Human platelet derived extracellular vesicles from Cellarcus provide a consistent, rigorously characterized reference sample to baseline assays over multiple runs. These samples are also suitable for researchers examining platelet derived exosomes, microvesicles, or other EVs.

Cellarcus’ Human Platelet Derived Extracellular vesicles are a reference sample prepared from platelets obtained by a standard platelet enrichment protocol. Platelet vesiculation is induced by Ca2+ selective ionophore. The resulting vesicles are isolated via centrifugation and provided at a concentration of 1 x 107 vesicles/µL as measured by Vesicle Flow Cytometry (vFC™). Unlike traditional applications like NTA or TRPS, it is vesicle specific. The specificity of the measurement is a key driver behind 5-fold improved assay variability compared to these other applications. Concentrations measured by vFC™ are generally 1-2 log units lower than other applications because it is counting only vesicles, not all particles. vFC™ analysis enables characterization of these vesicles samples by size, concentration and cell surface cargo.

Available Sizes:50 µl 100 µl 500 µl
Species: Human
Source: Platelets from patient samples
Surface Molecule Expression Information: Positive: CD41, Phosphotidylserine (as labeled by Annexin V).
Below Limit of Detection: (none tested).
Test and Concentration Information:Human Platelet Derived Extracellular Vesicles are EVs prepared by ionophore treatment of washed PLTs. This vesicle reference preparation is provided at a concentration of ~1 x 107 vesicles/µL, as measured by Vesicle Flow Cytometry (VFC). PLT EVs are a unimodal population of EVs with a median diameter of ~140 nm and a range of 80-210 nm, as measured by VFC.
Vesicle Size: 140 nm (Range: 80-210nm)
Tested Applications: VFC, NTA (e.g. NanoSightTM), RPS (e.g. qNanoTM)

Application Notes:

Vesicle flow cytometry requires a sufficiently sensitive instrument and vesicle analysis procedure. We provide simple, accurate, reproducible vesicle staining assays for this purpose (coming soon). At 1 x 107 vesicles/µL this reference sample uses 5 µl/test for VFC analysis. The sample concentration requirement is the same for NTA or RPS, however, volumes required for assay on many instruments are up to 10-fold higher. As much as 50 µl of this EV reference sample may be required for these assays. Exact volume(s) of reagents should always be empirically determined.

Method of Preparation: Ionophore treatment of platelets followed by centrifugation to isolate vesicles.
Buffer: Well-filtered isotonic saline (e.g. PBS pH 7.4)
Storage Conditions: These vesicles are stable for up to 12 months when stored at -80°C. Vesicles may also be stored cold (e.g.4°C) for up to 2 weeks.
Safety Considerations: Material is derived from human blood. Please handle in accordance with CDC Biosafety Requirements and follow Universal Precautions for handling potentially infectious samples.

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50 µl, 100 µl, 500µl

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