vCAL™ RAINBOW NANOBEADS
vCal™ Rainbow nanoBeads are ~500 nm beads with three fluorescence intensities plus a blank that should be well-resolved in the FITC, PE, and PE/Cy5 channels. These beads may be used to QC your flow cytometer when performing fluorescence detection in vesicle immunostaining like vFC™. Additionally, they are cross-calibrated to enable instrument calibration and reporting of immunofluorescent measurements in MESF units for FITC, PE, and PE/Cy5 surface cargo staining. See protocols below for more information.
In general, for smaller vesicles it is important that an instrument is able to resolve all peaks. For larger vesicles or very abundantly expressed proteins, inability to resolve the dim peak from the blank may not be required.
Bead Population(s): 4 populations with different fluorescence intensities ranging from 0 to >2000 MESF units, calibrated using commercially available standards. See lot specific information for details.
Concentration: 1×106 beads per ml. We recommend using 100 µl per well if running in 96 well plates.
Bead Size: 500nm
Buffer: 0.1 µm filtered HBSS containing 0.05% Azide.
Storage Conditions: Store Refrigerated (4°C).
1 ml (10 tests)