HUMAN RBC DERIVED EXTRACELLULAR VESICLES
$299.00 – $1,389.00
397 in stock
Source:Human Red Blood Cells
Vesicle Size:140 nm (Range: 80-210nm)
Certificate of Analysis
Are these the right vesicles for my application?
Human red blood cell derived vesicles from Cellarcus provide a consistent, rigorously characterized reference sample to baseline assays over multiple runs. They are suited for hematology researchers, but also provide a consistent, well characterized reference sample of exosomes and microvesicles (sometimes just described as “exosomes” by other manufacturers).
Cellarcus’ Human red blood cell derived EVs are prepared from red blood cells obtained from a local blood bank. RBC vesiculation is induced by Ca2+ selective ionophore. Vesicles are isolated from cells and debris via centrifugation and provided at a concentration of 1 x 107 vesicles/µL as measured by Vesicle Flow Cytometry. VFC analysis enables characterization of these vesicles by both size and cell surface cargo. This assay also specifically measures vesicle concentration ensuring 5-fold or greater lot-to-lot consistency vs other manufacturers vesicle preps.
Below Limit of Detection: (none tested).
Vesicle flow cytometry requires a sufficiently sensitive instrument and vesicle analysis procedure. We provide simple, accurate, reproducible vesicle staining assays for this purpose (coming soon). At 1 x 107 vesicles/µL this reference sample uses 5 µl/test for VFC analysis. The sample concentration requirement is the same for NTA or RPS, however, volumes required for assay on many instruments are up to 10-fold higher. As much as 50 µl of this EV reference sample may be required for these assays. Exact volume(s) of reagents should always be empirically determined.
50 µl, 100 µl, 500µl