SKU: CBS1-1
LIPO50™ SYNTHETIC VESICLE SIZE STANDARD

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Summary

Source:Synthetic
Vesicle Size:120 nm (Range: 80-140nm)
About Our Standard Package Sizes
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$239.00$629.00

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Are these the right standards for my application?

For sizing vesicles like exosomes or microvesicles via standard methods such as NTA, RPS, or flow cytometry, Synthetic Vesicle Size Standards provide a well characterized synthetic vesicle population for assessing the accuracy of the vesicle size calculations. These 50nm standards are suitable as a control in experiments looking at the lower end of the vesicle size spectrum as vesicle diameter typically range from approximately 50nm to over 1 micron depending upon vesicle type, source, and other characteristics.

Vesicle size distribution determination is important for characterizing vesicles. Size distribution provides an important paramater in QC of therapeutic vesicle preps. The size of vesicles is also physiologically relevant and is a determinant of vesicle uptake and other functions prescribed to EVs. Conventional standards used in vesicle sizing are polystyrene beads. PS beads are very different in their composition compared to natural vesicles and these differences lead to large underestimatation of vesicles’ size by as much as 40x. Cellarcus’ Synthetic Vesicle Size Standard is made of lipid vesicles called liposomes. These liposomes are prepared with lipid composition similar to that of a mammalian cell, extruded through a 100 nm nucleopore filter, and provided at a concentration of 1 x 107 vesicles/µL as measured by Vesicle Flow Cytometry. The vesicle standard is a unimodal vesicle population with a median diameter of ~120 nm and a range of ~80-140 nm (as measured by NTA and RPS).

Available Sizes: 20 Tests 40 Tests 80 Tests 4 x 20 Tests
Species: Synthetic (Appropriate size standard for vesicles from mammalian species)
Source: Synthetic
Surface Molecule Expression Information: Phosphotidylserine (PS) was incorporated during synthesis and confirmed via VFC testing with fluorescently labeled Annexin V.
Test and Concentration Information:Lipo50™ are supplied at a total of 5 x 105 vesicles per µl (as measured by VFC). This equates to 5 µl per test. A test is defined as the number of vesicles typically used in Vesicle Flow Cytometry.
Vesicle Size: 50 nm (Range: 30-60nm)
Tested Applications: Vesicle Flow Cytometry (VFC), NTA (e.g. NanoSightTM), RPS (e.g. qNanoTM)

Application Notes:

Vesicle flow cytometry requires a sufficiently sensitive instrument and vesicle analysis procedure. We provide simple, accurate, reproducible vesicle staining assays for this purpose (coming soon). Standards are titrated at 5 µl/test for VFC. For NTA or RPS, the same concentration of sample is generally sufficient, however, volumes required for assay on many instruments are up to 10-fold higher. As much as 50 µl of synthetic vesicles in solution may be required to standardize this approach when estimating vesicle sizes. Exact volume(s) of reagents should always be empirically determined.

Method of Preparation: 50 nm nucleopore SEC
Buffer: Well-filtered isotonic saline (e.g. PBS pH 7.4)
Storage Conditions: Synthetic Vesicle Size Standards are stable for up to 12 months when stored at -80°C. Vesicles may also be stored cold (e.g.4°C) for up to 2 weeks.
Safety Considerations: Gloves should be worn at all times when handling this product.
Size

20 Tests, 40 Tests, 80 Tests, 4 x 20 Tests

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