LIPO50™ SYNTHETIC VESICLE SIZE STANDARD
$239.00 – $629.00
800 in stock
Vesicle Size:120 nm (Range: 80-140nm)
Certificate of Analysis
Are these the right standards for my application?
For sizing vesicles like exosomes or microvesicles via standard methods such as NTA, RPS, or flow cytometry, Synthetic Vesicle Size Standards provide a well characterized synthetic vesicle population for assessing the accuracy of the vesicle size calculations. These 50nm standards are suitable as a control in experiments looking at the lower end of the vesicle size spectrum as vesicle diameter typically range from approximately 50nm to over 1 micron depending upon vesicle type, source, and other characteristics.
Vesicle size distribution determination is important for characterizing vesicles. Size distribution provides an important paramater in QC of therapeutic vesicle preps. The size of vesicles is also physiologically relevant and is a determinant of vesicle uptake and other functions prescribed to EVs. Conventional standards used in vesicle sizing are polystyrene beads. PS beads are very different in their composition compared to natural vesicles and these differences lead to large underestimatation of vesicles’ size by as much as 40x. Cellarcus’ Synthetic Vesicle Size Standard is made of lipid vesicles called liposomes. These liposomes are prepared with lipid composition similar to that of a mammalian cell, extruded through a 100 nm nucleopore filter, and provided at a concentration of 1 x 107 vesicles/µL as measured by Vesicle Flow Cytometry. The vesicle standard is a unimodal vesicle population with a median diameter of ~120 nm and a range of ~80-140 nm (as measured by NTA and RPS).
Vesicle flow cytometry requires a sufficiently sensitive instrument and vesicle analysis procedure. We provide simple, accurate, reproducible vesicle staining assays for this purpose (coming soon). Standards are titrated at 5 µl/test for VFC. For NTA or RPS, the same concentration of sample is generally sufficient, however, volumes required for assay on many instruments are up to 10-fold higher. As much as 50 µl of synthetic vesicles in solution may be required to standardize this approach when estimating vesicle sizes. Exact volume(s) of reagents should always be empirically determined.
20 Tests, 40 Tests, 80 Tests, 4 x 20 Tests