April 2020 Lab Roots Webinar

In this tutorial, Dr. John Nolan will describe fluorescence based EV size measurement. In this talk we will describe vFC™, an approach to characterizing EVs against the background of the current MISEV guidelines. We will go through a typical workflow including characterizing your instrument, running a vesicle size standard, and calibrating fluorescence measurements into surface area (and diameter). We will wrap up with a comparison to other approaches and a few recommendations on integrating methods for rigorous, reproducible measurements.