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18:15
In this tutorial, Dr. John Nolan will describe fluorescence based EV size measurement. In this talk we will describe vFC™, an approach to characterizing EVs against the background of the current MISEV guidelines. We will go through a typical workflow including characterizing your instrument, running a vesicle size standard, and calibrating fluorescence measurements into surface area (and diameter). We will wrap up with a comparison to other approaches and a few recommendations on integrating methods for rigorous, reproducible measurements.