Select a tetraspanin marker to comply with MISEV guidelines which recommend confirming the presence of an EV associated protein. Tetraspanins in the antibody cocktail are heterogeneously expressed, but present on most EVs (see example). Use the PE cocktail when sizing and counting EVs. When also using the kit to support no-wash immunofluorescent EV cargo measurement, select another conjugate to enable proper panel design. For instance, PE-Cy7 is useful beause it frees up the PE channel and other good channels on the instrument that detect bright, widely available antibody conjugates to enable sensitive quantitation of experimentally important proteins (including our vTag™ mAbs, validated for vFC™, searchable below).