vTAG™ ANTI-HUMAN CD11b ANTIBODY
NO WASH, QUANTITATIVE CD11b MEASUREMENT BY vFC™
$35.00 – $105.00
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Conjugate(s): PE
Est. Limit of Detection: 30 Molecules per EV
Cargo analysis using vTag™ antibodies should be accompanied by a vesicle specific analysis method such as vFC™. vTag™ antibodies bundled with the vFC™ assay will be discounted 25%. |
Certificate of Analysis
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Description
CD9 Immunostaining in vFC™
vFC™ (#CBS4) is an assay that enables specific detection and characterization of individual extracellular vesicles such as exosomes and microvesicles on commercially available flow cytometers. This protocol makes use of vFC™ to provide a standardized means of analyzing individual vesicles to measure size, concentration, and CD11b expression simultaneously. Because vFC™ triggers on vesicles, pairing antibody staining with vFC™ enables a highly-reproducible, no-wash assay. In this protocol, vesicles will be stained using our vTag™ Anti-Human CD11b Antibody. See protocol 2 in the vFC™ kit handbook for additional considerations and full protocol details.Procedure
- Dilute sample containing EVs to approximately 5×106 extracellular vesicles per µl. To empirically determine vesicle concentration and identify an optimal pre-stain dilution, see supporting protocols included in the vFC™ kit.
- Prepare a working concentration of vFRed™ Red (5 µl per well) according to kit instructions
- In duplicate, in the included 96 well plate, add 35 µl of dilution buffer, 5µl of vFRed™ Red, 5 µl of vTag™ anti-human CD11b antibody, and 5 µl of EV sample.
- Incubate in the dark for 1hr.
- Perform serial dilution of the samples as described in the assay handbook. Briefly, samples are to be diluted from between 1:100 to 1:1000 from staining concentration depending upon the flow cytometer being used.
- Analyze the diluted sample of labeled EVs on a qualified flow cytometer.
CD11b (also known as Integrin αM or Mac-1) is an integrin expressed primarily on myeloid cells such as monocytes, neutrophils, and macrophages. It plays a crucial role in leukocyte adhesion, migration, and phagocytosis by interacting with various ligands, including ICAM-1 and complement component iC3b. CD11b is essential for innate immune responses, facilitating the clearance of pathogens and cellular debris. (see the data by vesicle origin resource for more info).
MSDS-
vFC™ VESICLE FLOW CYTOMETRY EV ANALYSIS ASSAY
$450.00 – $2,100.00 Select options This product has multiple variants. The options may be chosen on the product page
FOR COUNTING AND SIZING VESICLES IN BIOFLUIDS, MEDIA, OR BUFFER -
HUMAN PLATELET DERIVED EXTRACELLULAR VESICLES
$275.00 – $1,300.00 Select options This product has multiple variants. The options may be chosen on the product page
Additional information
| Size | 20 Tests, 100 Tests |
|---|---|
| Conjugate |
NO WASH, QUANTITATIVE CD11b MEASUREMENT BY vFC™”
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