Size Calibration

Vesicle Size Calibration (v317)

Calibration Inputs

Input Size Histogram Data

Input size standard data through one of the input methods listed below. It is recommended and easiest to select one from the dropdown menu. The Size standard will be used to calibrate vFred flourescence intensity so that size can be estimated from vFred.

Choose Input Method:

Input Fluorescence Histogram

Load data from FCS Express histogram. To get the histogram data right click on the gated vFred flourescence histogram and when a menu comes up go to copy overlay data then click on overlay 1. The flourescence histogram as well as the size standard will be used to generate coefficients that will be used to estimate the size of samples.

Choose Input Method:

Calibration Results

Advanced Display

✔ Success

Your Calibration was successful. Copy the slope and y-intercept into FCS Express Parameter Math. The template will now be able to estimate size for vesicles using vFred flourescence.

X Failure

Your Calibration failed. Your r-squared was too low.

Results

r2: 

slope: 

y-intercept: 

Diameter Data

Histogram Data
Size Count
Statistics
Stat Value

Surface Area Data

Histogram Data
Surface Area Count
Statistics
Stat Value

VFC Fluorescence Data

Histogram Data
IntensityCount
Statistics
Stat Value

Regression Information

Linear Regression: y=mx+b
Regression used to estimate surface area using Fluorescence.
Statistics
XY
equation: 
slope (m): 
y-int (b): 
r2: 
Use the slope and intercept values in FCS Express Parameter Math

Calibrated Surface Area Data

Histogram Data
Size Count
Statistics
Stat Value

Calibrated Diameter Data

Histogram Data
Size Count
Statistics
Stat Value
0